MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods

Autores

  • Max Roberto Batista Araújo Instituto Hermes Pardini S/A
  • Luisa Ferreira Seabra Núcleo Técnico Operacional, Setor de Microbiologia, Instituto Hermes Pardini. Vespasiano, MG, Brasil.
  • Mireille Ângela Bernardes Sousa Núcleo Técnico Operacional, Setor de Microbiologia, Instituto Hermes Pardini. Vespasiano, MG, Brasil.

Palavras-chave:

Mass spectrometry, matrix-assisted laser desorption-ionization, candidiasis, Candida, yeasts

Resumo

Introduction: Identification of yeast species has clinical and epidemiological value. Different methods can be used, such as chromogenic media, microculture on corn meal agar with Tween 80, as well as conventional biochemical and automated methods. Recently, proteomic studies employing matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry have been a major advance in diagnosis due to speed of execution and accuracy of results. Methods: For this study, 79 yeast samples were submitted to identification using chromogenic medium, microculture on corn meal-Tween 80 agar, VITEK® 2 Compact identification, and MALDI-TOF mass spectrometry. Results: Most of the 79 samples were identified, with differences in the performance of the methods used. Colonial morphology and microscopy were compatible with the genus Candida. MALDI-TOF mass spectrometry had the best performance, with 78 strains identified (98.7%), compared to VITEK® 2 Compact (92.4%) and microculture on corn meal agar (70.9%). Conclusion: MALDI-TOF mass spectrometry using the VITEK® MS instrument performed best and has proven to be a revolutionary method in clinical microbiology laboratories. Regarding the identification of C. albicans and C. tropicalis, the chromogenic medium had excellent performance, thus being a good option to optimize the process.

 

Keywords: Mass spectrometry; matrix-assisted laser desorption-ionization; candidiasis; Candida; yeasts

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Biografia do Autor

Max Roberto Batista Araújo, Instituto Hermes Pardini S/A

Setor de Microbiologia. Núcleo Técnico Operacional.

Luisa Ferreira Seabra, Núcleo Técnico Operacional, Setor de Microbiologia, Instituto Hermes Pardini. Vespasiano, MG, Brasil.

Setor de Microbiologia. NTO. Hermes Pardini

Mireille Ângela Bernardes Sousa, Núcleo Técnico Operacional, Setor de Microbiologia, Instituto Hermes Pardini. Vespasiano, MG, Brasil.

Setor de Microbiologia. NTO. Hermes Pardini

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Publicado

2019-08-20

Como Citar

1.
Araújo MRB, Seabra LF, Bernardes Sousa M Ângela. MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods. Clin Biomed Res [Internet]. 20º de agosto de 2019 [citado 5º de dezembro de 2022];39(2). Disponível em: https://www.seer.ufrgs.br/index.php/hcpa/article/view/91902

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