Use of MALDI-TOF MS in diagnostic microbiology for identification of species that conventional methods usually fail to identify

Autores

  • Max Roberto Batista Araújo Instituto Hermes Pardini S/A
  • Luan Freitas Lana Universidade Federal de Minas Gerais.
  • Luisa Ferreira Seabra Instituto Hermes Pardini

Palavras-chave:

Non-tuberculous Mycobacteria, Mycobacterium abscessus, Bacterial infections, Actinotignum schaalii, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Resumo

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in clinical diagnostic laboratories for the identification of microorganisms. It has a relevant advantage compared to other methods in terms of speed to provide results, being an alternative for addressing restrictions in clinical diagnosis as it may replace or complement existing identification techniques. This is especially important because some rare microorganisms would be identified only by higher cost techniques which are not widely available, such as genetic sequencing. Thus, the present paper reports two cases in which uncommon microorganisms were identified effectively and quickly.

Keywords: Non-tuberculous Mycobacteria; Mycobacterium abscessus; Bacterial infections; Actinotignum schaalii; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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Biografia do Autor

Max Roberto Batista Araújo, Instituto Hermes Pardini S/A

Setor de Microbiologia. Núcleo Técnico Operacional.

Luan Freitas Lana, Universidade Federal de Minas Gerais.

Unidade de Apoio Operacional, Hospital das Clínicas.

Luisa Ferreira Seabra, Instituto Hermes Pardini

Setor de Microbiologia. Núcleo Técnico Operacional.

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Publicado

2019-08-20

Como Citar

1.
Araújo MRB, Lana LF, Seabra LF. Use of MALDI-TOF MS in diagnostic microbiology for identification of species that conventional methods usually fail to identify. Clin Biomed Res [Internet]. 20º de agosto de 2019 [citado 29º de novembro de 2022];39(2). Disponível em: https://www.seer.ufrgs.br/index.php/hcpa/article/view/90933

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