Glossitis by Actinobacillus equuli subsp. equuli in an Equine

Authors

  • Hugo Shisei Toma Departamento de Medicina Veterinária, Universidade Federal de Lavras (UFLA), Lavras, MG, Brazil.
  • Mariana Bueno Carvalho Departamento de Medicina Veterinária, Universidade Federal de Lavras (UFLA), Lavras, MG, Brazil.
  • Armando de Mattos Carvalho Departamento de Medicina Veterinária, Universidade Federal de Lavras (UFLA), Lavras, MG, Brazil.
  • Raquel Aparecida Sales da Cruz Departamento de Medicina Veterinária, Universidade Federal de Lavras (UFLA), Lavras, MG, Brazil.
  • Fábio Bernardo Schein Departamento de Medicina Veterinária, Universidade Federal de Lavras (UFLA), Lavras, MG, Brazil.
  • Kamila Peruchi Fernandes Martins Departamento de Medicina Veterinária, Universidade Federal de Lavras (UFLA), Lavras, MG, Brazil.
  • Claudia Dias Monteiro Toma Departamento de Medicina Veterinária, Universidade Federal de Lavras (UFLA), Lavras, MG, Brazil.
  • Luciano da Silva Cabral Departamento de Medicina Veterinária, Universidade Federal de Lavras (UFLA), Lavras, MG, Brazil.

DOI:

https://doi.org/10.22456/1679-9216.97970

Abstract

Background:Actinobacillosis in adult horses is rare, and is often associated with infection due to Actinobacillus equuli subsp. equuli, which is a commensal organism inhabiting the oral cavity of healthy animals. It is an opportunistic pathogen, and the infection usually occurs secondary to another primary disease or due to predisposing factors such as pre-existing lesions, mainly in the oral cavity. Infection may be associated with peritonitis, bacterial endocarditis, pneumonia, enteritis, infectious periorchitis, abortion, and septicemia. This paper aims to describe the clinical, microbiological, and pathological aspects of actinobacillosis in the oral cavity of a horse.

Case:A case of actinobacillosis has been reported in a 22-year-old gelding Quarter Horse. According to the owner, the animal presented with progressive weight loss during the previous three months, and leakage of food from the oral cavity. Examination of the oral cavity revealed sharp enamel overgrowths, which had been corrected during a previous dental procedure. However, five days after the procedure, the animal developed halitosis. Ceftiofur sodium (4.4 mg/kg intramuscularly, every 24 h) was prescribed; two days after the commencement of antimicrobial therapy, the animal presented with sialorrhea, bilateral submandibular lymphadenomegaly, protrusion of the tongue, edema of the base of the tongue, cheeks, and gums, in addition to black-colored sores and scales in the oral mucosa, particularly affecting the base of the tongue and gums. Samples from ulcerative lesions found at the base of the tongue and gums were collected using sterile swabs, and culture and antibiogram were requested. Microbiological culture led to the growth of small colonies measuring less than 5 mm in diameter, that were non-hemolytic, slightly whitish in color but non-opaque, smooth and bright in appearance. These were identified as gram-negative rods on microscopy, and further characterized as Actinobacillus equuli subsp. equuli based on the phenotypic and biochemical findings. The antibiogram revealed sensitivity of the organisms to cephalothin and doxycycline, intermediate sensitivity to amoxicillin, and resistance to florfenicol, metronidazole, clindamycin, and sulfazotrim. The complete blood count revealed anemia with a reduction in hemoglobin (10.1 g/dL) and hematocrit (27.9%), neutrophilia (10.670 x 10³ /mm³), and lymphopenia (330 x 10³ /mm³). Decrease in serum albumin (2.0 g/dL) and alkaline phosphatase (91 IU/L), and increase in globulin (4.9 g/dL), aspartate aminotransferase (361 4 IU/L), and urea (123.8 mg/dL) were noted on biochemical analyses. Two days after the onset of acute clinical signs, the animal died; necropsy revealed severe, subacute, fibronecrotic glossitis associated with myriads of basophilic bacteria.

Discussion:This report describes an infection of the oral cavity by Actinobacillus equuli subsp. equuli following a dental procedure, a finding compatible with the opportunistic nature of these bacteria. The presence of enamel overgrowths resulted in the formation of traumatic lesions on the oral mucosa and tongue that favored colonization of the bacteria. The case of equine glossitis reported in this study had a similar clinical presentation to the classic actinobacillosis in cattle, also known as “wooden tongue”, an infection caused by Actinobacillus lignieresii; common symptoms in cattle include oral edema, sialorrhea, and dysphagia.


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References

Aalbaek B., Ostergaard S., Buhl R., Jensen H. E., Christensen H. & Bisgaard M. 2007. Actinobacillus equuli subsp. equuli associated with equine valvular endocarditis: Case report. Acta Pathologica, Microbiologica, et Immunologica Scandinavica. 115(12): 1437-1442.

Albornoz L. & Sali G. 2012. Reporte de un caso de actinobacilosis enzoótica en vaquillonas Holando em sistema pastoril. Veterinaria (Montevideo). 48(188): 29-31.

Andreazza D., Wouters A.T.B., Watanabe T.T.N., Boabaid F.M., Wouters F., Souza F.S., Souza S.O. & Driemeier D. 2013. Caracterização patológica e imuno-histoquímica das lesões de actinobacilose em bovinos. Pesquisa Veterinária Brasileira. 33(3): 305-309.

Bailey G.D. & Love D.N. 1991. Oral associated bacterial infection in horses: studies on the normal anaerobic flora from the pharyngeal tonsillar surface and its association with lower respiratory tract and paraoral infections. Veterinary Microbiology. 26(4): 367-379.

Bisgaard M., Piechulla K., Ying Y.T., Frederiksen W. & Mannheim W. 1984. Prevalence of organisms described as Actinobacillus suis or haemolytic Actinobacillus equuli in the oral cavity of horses. Comparative investigations of strains obtained and porcine strains of A. suis sensu stricto. Acta Pathologica Microbiologica Scandinavica Series B: Microbiology. 92(6): 291-298.

Carvalho A.M., Toma H.S., Artman T.A., Spor K.A.H., Pinheiro J.C.M.N. & Camargo L.M. 2015. Actinobacilose em bovino secundária a descorna cosmética: relato de caso. Veterinária e Zootecnia. 22(1): 32-36.

Casey M. 2013. A new understanding of oral and dental pathology of the equine cheek teeth. Veterinary Clinics of North America: Equine Practice. 29(2): 301-324.

Christensen H. & Bisgaard M. 2004. Revised definition of Actinobacillus sensu stricto isolated from animals: A review with special emphasis on diagnosis. Veterinary Microbiology. 99(1): 13-30.

Christensen H., Bisgaard M. & Olsen J.E. 2002. Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or Bisgaard taxon 11 and proposal of A. equuli subsp. equuli subsp. nov. and A. equuli subsp. haemolyticus subsp. nov. International journal of systematic and evolutionary microbiology. 52(5): 1569-1576.

Cockerill F.R., Wikler M.A., Alder J., Dupley M.N., Eliopoulous G.M., Ferraro M.J., Hardy D.J., Hecht D.W., Hindler J.A., Patel J.B., Powell M., Swenson, J.M., Thomson R.B., Traczewski M.M., Turnidge J.D., Weinstein M.P. & Zimmer B.L. 2012. Performance standards for antimicrobial susceptibility testing. Twenty-Second Informational Supplement. CLSI document M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute. 32(3):1-188.

Dixon P.M., du Toit N & Dacre I.T. 2011. Equine dental pathology. In: Easley J., Dixon P.M. & Schumacher J. (Eds). Equine Dentistry. 3rd edn. London: Elsevier, pp.129-147.

Donahue J.M., Sells S.F. & Bolin D.C. 2006. Classification of Actinobacillus spp. isolates from horses involved in mare reproductive loss syndrome. American Journal of Veterinary Research. 67(8): 1426-1432.

Fuller M.C. & Abutarbush S.M. 2007. Glossitis and tongue trauma subsequent to administration of an oral medication, using an udder infusion cannula in a horse. The Canadian Veterinary Journal. 48(8): 845-847.

Huang B.F., Kropinski A.M., Bujold A.R. & MacInnes J.I. 2015. Complete genome sequence of Actinobacillus equuli subspecies equuli ATCC 19392(T). Standards in genomic sciences. 10(32): 1-7.

Layman Q.D., Rezabek G.B., Ramachandran A., Love B.C. & Confer A.W. 2014. A retrospective study of equine actinobacillosis cases: 1999-2011. Journal of Veterinary Diagnostic Investigation. 26(2): 365-375.

Loss D.E., Vogg G., Bandarra P.M., Raymundo D.L., Conceição E.O., Dalto A.G.C., Oliveira M.B. & Driemeier D. 2006. Ocorrência em surto de actinobacilose em bovinos mantidos em area de capim anoni (Eragrostis plana) no Rio Grande do Sul. In: 2º Encontro Nacional de Laboratórios de Diagnóstico Veterinário (Campo Grande, Brazil). p.41.

Matthews S., Dart A., Dowling B., Hodgson J. & Hodgson D. 2001. Peritonitis associated with Actinobacillus equuli in horses: 51 cases. Australian Veterinary Journal. 79(8): 536-539.

Meyer W., Kacza, J., Schnapper A., Verspohl J., Hornickel I. & Seeger J. 2010. A first report on the microbial colonisation of the equine oesophagus. Annals of Anatomy-Anatomischer Anzeiger. 192(1): 42-51.

Moore R., Schneider R.K., Kowalski J., Bramlage L.R., Mecklenburg L.M. & Kohn C.W. 1992. Antimicrobial susceptibility of bacterial isolates from 233 horses with musculoskeletal infection during 1979–1989. Equine Veterinary Journal. 24(6): 450-456.

Pagliosa G.M., Alves G.E.S., Faleiros R.R., Saliba E.O.S., Sampaio I.B.M., Gomes T.L.S., Gobesso A.A.O. & Fantini P. 2006. Influence of excessive enamel points on nutrients digestibility in diets of horses. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 58(1): 94-98.

Patterson-Kane J.C., Donahue J.M. & Harrison L.R. 2001. Septicemia and peritonitis due to Actinobacillus equuli infection in an adult horse. Veterinary Pathology. 38(2): 230-232.

Quinn P.J., Carter M.E., Markey B. & Carter G.R. 1994. Clinical Veterinary Microbiology. London: Mosby-Wolfe, 648p.

Reed S.M., Bayly W.M. & Sellon D.C. 2017. Equine Internal Medicine. 4th edn. St. Louis: Elsevier, 1566p.

Rycroft A.N. & Garside L.H. 2000. Actinobacillus Species and their Role in Animal Disease. The Veterinary Journal. 159(1): 18-36.

Smith M.A. & Ross M.W. 2002. Postoperative infection with Actinobacillus spp. in horses: 10 cases (1995-2000). Journal of the American Veterinary Medical Association. 221(9): 1306-1310.

Snyder J.R., Pascoe J.R. & Hirsh D.C. 1987. Antimicrobial susceptibility of microorganisms isolated from equine orthopedic patients. Veterinary Surgery. 16(3): 197-201.

Sternberg S. 1998. Isolation of Actinobacillus equuli from the oral cavity of healthy horses and comparison of isolates by restriction enzyme digestion and pulsed-field gel electrophoresis. Veterinary Microbiology. 59(2-3): 147-156.

Uzal F.A., Plattner B.L. & Hostetter J.M. 2016. Alimentary system. In: Maxie M.G. (Ed). Jubb, Kennedy and Palmer’s Pathology of Domestic Animals. 6th edn. Philadelphia: Saunders Elsevier, pp.1-19.

Published

2020-01-01

How to Cite

Shisei Toma, H., Carvalho, M. B., Carvalho, A. de M., Sales da Cruz, R. A., Schein, F. B., Fernandes Martins, K. P., Monteiro Toma, C. D., & Cabral, L. da S. (2020). Glossitis by Actinobacillus equuli subsp. equuli in an Equine. Acta Scientiae Veterinariae, 48. https://doi.org/10.22456/1679-9216.97970