Mammary Gland Malignant Myoepithelioma in a Domestic Rabbit (Oryctolagus cuniculus)

Authors

  • Halana do Carmo Silva Departamento de Medicina Veterinária (DMV), Universidade Vila Velha (UVV), Vila Velha, ES, Brazil.
  • Avisa Rodrigues de Oliveira Departamento de Medicina Veterinária (DMV), Universidade Vila Velha (UVV), Vila Velha, ES, Brazil.
  • Rodrigo dos Santos Horta Departamento de Medicina Veterinária (DMV), Universidade Vila Velha (UVV), Vila Velha, ES, Brazil.
  • Betânia Souza Moereira Departamento de Medicina Veterinária (DMV), Universidade Vila Velha (UVV), Vila Velha, ES, Brazil.
  • Tatiany Luiza Silveira Departamento de Medicina Veterinária (DMV), Universidade Vila Velha (UVV), Vila Velha, ES, Brazil.
  • Giovanni Dantas Cassali Departamento de Medicina Veterinária (DMV), Universidade Vila Velha (UVV), Vila Velha, ES, Brazil.
  • Mayra Cunha Dantas Departamento de Medicina Veterinária (DMV), Universidade Vila Velha (UVV), Vila Velha, ES, Brazil.

DOI:

https://doi.org/10.22456/1679-9216.92568

Abstract

Background: Malignant myoepithelioma is a rare neoplasm in humans and rats, poorly reported in dogs and not previously described in rabbits. This study aimed to report a case of malignant mammary myoepithelioma in a domestic rabbit (Oryctolagus cuniculus). Case: A domestic, hybrid, female, entire, nulliparous, seven-year-old rabbit was attended in the Veterinary Hospital Prof. Ricardo Alexandre Hippler at Universidade Vila Velha (UVV) with a history of apathy, lethargy and anorexia. In the clinical examination a 5 cm tumour was observed in the right abdominal mammary gland (M2), along with floating smaller lesions in M1 and M3. There were no palpable abnormalities in the axillary and inguinal lymph nodes. Complete blood count and serum biochemistry were within the normal range. Fine needle aspiration was performed for cytopathological evaluation of the tumour in M2 and it was suggestive of a malignant glandular neoplasm, with probable epithelial origin. Complete staging included chest and abdominal radiographs, but they were unremarkable. The patient was submitted to a right unilateral mastectomy, involving resection of the inguinal lymph node. The sample was sent to the Laboratory of Animal Pathology of UVV for routine histological evaluation. Histopathological evaluation in M2 was compatible with malignant myoepithelioma based on the literature, while nodules in M1 and M3 were classified as mammary cysts. The inguinal lymph node showed no significant abnormalities. An immunohistochemical panel was performed in the Comparative Pathology Laboratory of the Institute of Biological Sciences of the Universidade Federal de Minas Gerais (UFMG), using immunomarkers (pan-cytokeratin, p63 and vimentin) to characterize the tumour and confirm its diagnosis. Immunohistochemistry revealed expression of p63, and was negative for pan-cytokeratin and vimentin, which confirmed the myoepithelial origin, without involvement of the epithelial compartment. Clinical follow-up was recommended every 2-3 months, including abdominal ultrasonography, chest X-rays, complete blood count and serum biochemistry. Ultrasonography showed thinning of the uterine wall, dilation of 3.7 cm in diameter and presence of endometrial cysts of varied sizes, around 0.7 cm. Neutering was recommended but it was not elected by the pet´s responsible. These findings remained stable throughout the follow-up, which was greater than 405 days.Discussion: This is the first report of a malignant myoepithelioma in rabbit, with a detailed description of the clinical, surgical, pathological and immunophenotypic aspects of this neoplasm in this species. This is a tumour characterized by proliferation of epithelioid to spindle-like myoepithelial cells, as observed in this case, forming an extensive and well delimited solid area with marked central necrosis, compatible with what has already been described in the literature on bitches and women. As observed in this case, the neoplastic cells usually exhibit moderate cellular and nuclear pleomorphism, moderate anisocytosis and anisokaryosis, and several mitotic figures; different from what is observed in women, in which mitotic figures vary from small to moderate amount. Neoplasia and mammary cysts were removed by mastectomy, and the owner choose not to castrate the animal. On ultrasonography, after 12 months, the endometrium presented structures similar to cysts and was hyperplastic, which is commonly reported in association to mammary neoplasia. Nervertheless, the pet´s owner choose not to spay the rabbit and the lesions were stable on imaging follow-up

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References

Asakawa M.G., Goldschmidt M.H., Une Y. & Nomura Y. 2008. The immunohistochemical evaluation of estrogen receptor-α and progesterone receptors of normal, hyperplastic, and neoplastic endometrium in 88 pet rabbits. Veterinary Pathology. 45(2): 217-225.

Bauchet A.L., Elies L., Maliver P., Fouque M.C., Balme E., Château-Joubert S., Schorsch F. & Fontaine J.J. 2008. A mammary gland adenomyoepithelioma in a C57BL/6 mouse. Experimental and Toxicologic Pathology. 60(4-5): 307-311.

Baum B. & Hewicker-Trautwein M. 2015. Classification and Epidemiology of Mammary Tumors in Pet Rabbits (Oryctolagus cuniculus). Journal of Comparative Pathology. 152(4): 291-298.

Cassali G.D., Lavelle G.E., De Nardi A.B., Ferreira E., Bertagnolli A.C., Estrela-Lima A., De Nardi A.B., Ghever C., Sobral R.A., Amorim R.L., Oliveira L.O., Sueiro F.A.R., Beserra H.E.O., Bertagnolli A.C., Gamba C.O., Damasceno K.A., Campos C.B., Araujo M.R., Campos L.C., Monteiro L.N., Nunes F.C., Horta R.S., Reis D.C., Luvizotto M.C.R., Magalhães G.M., Raposo J.B., Ferreira A.M.R., Tanaka N.M., Grandi F., Ubukata R., Batschinski K., Terra E.M., Salvador R.C.L., Jark P.C., Delecrodi J.E.R., Nascimento N.A., Silva D.N., Silva L.P., Ferreira K.C.R.S., Frehse M.S., Santis G.W., Silva E.O., Guim T.N., Kerr B., Cintra P.P., Silva F.B.F., Leite J.S., Mello M.F.V., Ferreira M.L.G., Fukumasu H., Salgado B.S. & Torres R. 2014. Consensus for the diagnosis, prognosis and treatment of canine mammary tumours. Brazilian Journal Veterinary Pathology. 7(2): 38-69.

Couto S.E.R. 2002. Criação e manejo de coelhos. In: Andrade A., Pinto S.C. & Oliveira R.S. (Eds). Animais de Laboratório: criação e experimentação [online]. Rio de Janeiro: FIOCRUZ, pp.98-99.

Faustino A.M. & Pereira P.D. 2007. A salivary malignant myoephitelioma in a dog. Veterinary Journal. 173: 223-226.

Gama A., Alves A., Gartner F. & Schimit F. 2003. p63: A Novel Myoepithelial Cell Marker in Canine Mammary Tissues. Veterinary Pathology. 40(4): 412-420.

Goldschmidt M., Penã L., Rasotto R. & Zappulli V. 2011. Classification and Grading of Canine Mammary Tumors. Veterinary Pathology. 48(1): 117-131.

Greene H.S.N. & Strauss J.S. 1949. Multiple primary tumors in the rabbit. Cancer. 2(4): 673–691.

Greene H.S.N. 1939. Familial mammary tumors in the rabbit: I. clinical history, II. Gross and microscopic pathology. Journal of Experimental Medicine. 70(2): 147-166.

Harcourt-Brown F. 2002. Urogenital diseases. In: Varga M. (Ed). Textbook of rabbit medicine. Woburn, Massachusetts: Reed Educational and Professional Publishing, pp.350-351.

Künzel F., Grinninger P., Shibly S., Hassan J., Tichy A., Berghold P. & Fuchs-Baumgartinger A. 2015. Uterine disorders in 50 pet rabbits. Journal of the American Animal Hospital Association. 51(1): 8-14.

Lakhani S.R., O´Hare M.J., Monaghan P., Winehouse J., Gazet J.C. & Sloane J.P. 1995. Malignant myoepithelioma (Myoepithelioma carcinoma) of the breast: a detailed cytokeratin study. Journal of Clinical Pathology. 48(2): 164-167.

Liang Y., Zeng J., Kang D., Wang L., Chen C., Xu J. & Wu Q. 2014. Malignant myoepithelioma of the breast: a case report and review of literature. International Journal of Clinical and Experimental Pathology. 7(5): 2654-2657.

Papazian M., Kalantzis I., Galanopoulos G., Mani I., Tzaida O., Iacovidou I. & Ziras N. 2016. Malignant myoepithelioma of the breast: A case report and review of the literature. Molecular and Clinical Oncology. 4(5): 723-727.

Percy D.H. & Barthold S.W. 2007. Rabbit. In: Pathology of Laboratory Rodents and Rabbits. 3rd edn. Ames: Blackwell, pp.310-313.

Rasotto R., Zappulli V., Castagnaro M. & Goldschmidt M.H. 2012. A Retrospective Study of Those Histopathologic Parameters Predictive of Invasion of the Lymphatic System by Canine Mammary Carcinomas. Veterinary Pathology. 49(2): 330-340.

Rodigheri S.M. 2016. Parâmetros da resposta metabólica ao trauma cirúrgico induzido por mastectomia unilateral associada ou não à ovariohisterectomia em cadelas. 82f. Jaboticabal, São Paulo. Dissertação (Doutorado em Cirurgia Veterinária) – Programa de Pós-Graduação em Cirurgia Veterinária, Universidade Estadual Paulista.

Saito K., Nakanishi M. & Hassegawa A. 2002. Uterine disorders diagnosed by ventrotomy in 47 rabbits. Journal of Veterinary Medical Science. 64(6): 495-497.

Schöniger S., Horn L.C. & Schoon H.A. 2014. Tumors and tumor-like lesions in the mammary gland of 24 pet rabbits: a histomorphological and immunohistochemical characterization. Veterinary Pathology. 51(3): 569-80.

Shahbazfar A.A., Mohammadpour H. & Isfahani H.R. 2012. Mammary Gland Carcinosarcoma in a New Zealand White Rabbit (Oryctolagus cuniculus). Acta Scientiae Veterinariae. 40(1): 1025.

Sikoski P., Trybus J., Cline J.M., Muhammad F.S., Eckhoff A., Tan J., Lockard M., Jolley T., Britt S. & Kock N.D. 2008. Cystic mammary adenocarcinoma associated with a prolactin-secreting pituitary adenoma in a New Zealand White rabbit (Oryctolagus cuniculus). Comparative Medicine. 58(3): 297-300.

Tan P.H. & Ellis I.O. 2013. Myoepithelial and epithelial-myoepithelial, mesenchymal and fibroepithelial breast lesions: updates from the WHO Classification of Tumors of the Breast 2012. Journal of Clinical Pathology. 66(6): 465-470.

Terada T. 2011. Malignant myoepithelioma of the breast. Pathology International. 61(2): 99-103.

Vinci A., Bacci B., Benazzi C., Caldin M. & Sarli G. 2010. Progesterone Receptor Expression and Proliferative Activity in Uterine Tumours of Pet Rabbits. Journal of Comparative Pathology. 142(4): 323-327.

Walter B., Poth T., Bohmer E., Braun J. & Matis U. 2010. Uterine disorders in 59 rabbits. Veterinary Record. 166(8): 230-233.

Published

2019-01-01

How to Cite

Silva, H. do C., de Oliveira, A. R., Horta, R. dos S., Moereira, B. S., Silveira, T. L., Cassali, G. D., & Dantas, M. C. (2019). Mammary Gland Malignant Myoepithelioma in a Domestic Rabbit (Oryctolagus cuniculus). Acta Scientiae Veterinariae, 47. https://doi.org/10.22456/1679-9216.92568

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