Antimicrobial Photodinamic Therapy Combined with Laser Photobiomodulation in the Treatment of Skin Wounds in a Dog (Canis lupus familiaris)

Authors

  • Fernando Alzamora Filho Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil. https://orcid.org/0000-0001-6456-8998
  • Manoel Luiz Ferreira Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil. https://orcid.org/0000-0002-7977-6383
  • Marcus Vinícius Alves da Silva Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil. https://orcid.org/0000-0002-1764-0798
  • Anna Claudia Mombrini Silva Barbosa Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil. https://orcid.org/0000-0001-7991-606X
  • Bruna Guedes Carvalho Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.
  • Marcel Vasconcellos Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil. https://orcid.org/0000-0003-0571-6588
  • José Marcus Raso Eulálio Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil. https://orcid.org/0000-0002-5486-0132
  • José Eduardo Ferreira Manso Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil. https://orcid.org/0000-0001-9694-7415

DOI:

https://doi.org/10.22456/1679-9216.116169

Abstract

Background: Laser photobiomodulation has been used in the treatment of various injuries and diseases. This promotes modulation of the inflammatory process, edema reduction and devitalized tissue regeneration. The advantages of Antimicrobial photodynamic therapy are its easy application and the absence of side effects. Other advantages are the cost of the therapy, minimal damage to animal tissue, the broad spectrum of action, and efficiency against strains resistant to antimicrobials. The aim of this study was to report the clinical and their resolution in a female dog with a traumatic, infected wound treated with laser phototherapy as an alternative therapy method.

Case: A 3-year-old bitch Border Collie, weighing 18 kg, from the municipality of Ilhéus, Bahia, Brazil, waspresented for examination with a history of traumatic laceration of the left thoracic member. On the anamnesis, it was reported that the patient presented with laceration of the left thoracic member. The wound was cleaned and an antibiotic [30 mg/kg of 12/12 h] and anti-inflammatory [0.1 mg/kg every 24 h were prescribed, both for 5 days]. Twenty-four h after the surgical procedure, there was dehiscence of the sutures, with daily topical therapy based on zinc oxide for secondary intention healing. Upon physical examination, the wound was found to be contaminated with swollen and erythematous edges, an ulcerated area with devitalized tissue, serous exudate, and 8.8 cm2 of wounded area. Given the macroscopic characteristics of the lesion, phototherapy was associated with conventional therapy until complete healing of the wound, with three weekly applications at 48 h intervals. Initially, the wound was cleaned with saline solution at 0.9% and a single treatment with aPDT was scheduled due to the high degree of contamination. The dosimetry parameters of irradiation were calculated according to the wounded area with a diode laser of 0.1W of power, continuous emission, spot area of 0.028 cm2, and energy of 9 J per application point. A gauze imbibed with 1 mL of methylene blue aqueous solution (300 μM), which was the photosensitizer was applied to the lesion, with a pre-irradiation time of 5 min, after which it was irradiated with red laser (RL) (λ = 660nm) for 90 s per point, using the sweeping technique. The edge of the lesion was irradiated with infrared laser (IRL) (λ = 808 nm), total energy of 5 J, using the technique of specific points and 1 J of energy/point. After aPDT, low-intensity laser therapy (LILT) sessions were set up with RL and IRL, with energies of 0.5 J/point and 1 J/point, respectively. The wound was cleaned daily, protected with a bandage, and clinically evaluated until complete regression.

Discussion: In the present case, methylene blue was used as a photosensitizer, but it is noteworthy that, apparently, each microorganism responds differently to photosensitizers. Thus, the therapy becomes specific for each application, for example: the type of photosensitizer, its concentration, pre-irradiation time, type of light used in photosensitization, wavelength, energy, power, mode delivery of light. Thus, for the best result, the specific protocol in each application should be used  Low-intensity laser therapy is an easily executed technique with effective results. The use of PDT associated with photobiomodulation therapy enabled rapid healing of the cutaneous wound, in addition to an improvement in clinical signs and pain caused by the lesion. The technique proved to be an efficient alternative in the treatment of wounds, whether used in isolation or associated with conventional therapy. 

Keywords: methylene blue, healing, wound, photobiomodulation, low-level laser.

Título: Terapia fotodinâmica antimicrobiana combinada com a fotobiomodulação a laser no tratamento de ferida cutânea em cão (Canis lupus familiaris).


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Author Biographies

Fernando Alzamora Filho, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.

Faculdade de Medicina Veterinária, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC). Rodovia Jorge Amado, km 16, Bairro Salobrinho, Ilhéus, Bahia, Brazil.

Manoel Luiz Ferreira, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.

Centro de Cirurgia Experimental, Departamento de Cirurgia, Faculdade de Medicina. Avenida Carlos Chagas Filho, 373 - Bloco K, 2º andar, Sala 32 - Cidade Universitária da Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

Marcus Vinícius Alves da Silva, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.

Centro de Cirurgia Experimental, Departamento de Cirurgia, Faculdade de Medicina. Avenida Carlos Chagas Filho, 373 - Bloco K, 2º andar, Sala 32 - Cidade Universitária da Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

Anna Claudia Mombrini Silva Barbosa, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.

Secretaria Estadual de Saúde do Espirito Santo (SESA-ES). Rua Engenheiro Guilherme José Monjardim Varejão, 225 Edíficio Enseada Plaza - Enseada do Suá, Vitória - ES, Brazil.

Bruna Guedes Carvalho, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.

Universidade do Grande Rio. Rua Professor José de Souza Herdy, 1160 - Jardim Vinte e Cinco de Agosto, Duque de Caxias, RJ, Brazil.

Marcel Vasconcellos, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.

Faculdade de Medicina - Centro Universitário Serra dos Órgãos. Avenida Alberto Tôrres, 111 - Alto, Teresópolis, RJ, Brazil. 

José Marcus Raso Eulálio, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.

Centro de Cirurgia Experimental, Departamento de Cirurgia, Faculdade de Medicina. Avenida Carlos Chagas Filho, 373 - Bloco K, 2º andar, Sala 32 - Cidade Universitária da Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

José Eduardo Ferreira Manso, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Ilhéus, BA, Brazil.

Centro de Cirurgia Experimental, Departamento de Cirurgia, Faculdade de Medicina. Avenida Carlos Chagas Filho, 373 - Bloco K, 2º andar, Sala 32 - Cidade Universitária da Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

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Published

2021-01-01

How to Cite

Filho, F. A., Ferreira, M. L., da Silva, M. V. A., Silva Barbosa, A. C. M., Carvalho, B. G., Vasconcellos, M., Eulálio, J. M. R., & Manso, J. E. F. (2021). Antimicrobial Photodinamic Therapy Combined with Laser Photobiomodulation in the Treatment of Skin Wounds in a Dog (Canis lupus familiaris). Acta Scientiae Veterinariae, 49. https://doi.org/10.22456/1679-9216.116169

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