Carcinoma with Vertebral Column Metastasis in Two Dogs




Background: Carcinomas are aggressive and invasive malignancies that originate from any type of epithelial cell and are responsible for many deaths in dogs. Carcinoma metastases occur primarily via the lymphatic route; however, they can occur by blood flow, thus reaching bone structures. In dogs, metastasis of mammary and squamous cell carcinomas to the skeletal system is poorly described. The aim of this study was to report two cases of dogs that developed metastases of carcinomas to the vertebral column.
Cases: Case 1. A 10 years-old, male, mixed-breed dog with paralysis of the left pelvic limb, subcutaneous mass in the lumbar region, apathy, anorexia and progressive weight loss and with a previous history of amputation of the right pelvic limb resulting from squamous cell carcinoma (SCC) in the integument of the cranial face of the femorotibiopatellar joint. Due to the patient's clinical condition and the negative prognosis associated with the neoplasia, euthanasia was performed. At necropsy, infiltrating the lumbar vertebrae from L5 to L7, a whitish and firm mass of approximately 15 x 8 cm was observed. Histologically, it comprised a malignant epithelial neoplastic development consisting of nests and cords interspersed with fibrovascular stroma, containing multiple keratine pearls. There was moderate to severe anisocytosis, severe anisokariosis, and about 4 mitosis figures for every 10 high power fields (400x). The histological features were consistent with an invasive SCC. Anti-cytokeratin and anti-p63 immunohistochemical (IHC) evaluations were performed, both with immunoreactivity in neoplastic cells. Case 2. An 8 years-old, female, Chihuahua with bilateral paralysis of the pelvic limbs, anorexia, and progressive weight loss, with a history of excision of solid mammary carcinoma. The clinical condition evolved to the absence of deep pain in all limbs and fecal and urinary retention, opting for euthanasia. At necropsy, a nodule between T3 and T4 was observed, yellowish-white and firm, measuring about 5 x 3.8 cm, invading the spinal canal, and compressing the spinal cord. Histologically, it comprised malignant epithelial neoplasia infiltrating intervertebral ligaments, musculature, and vertebrae of the thoracic spine, organized in a dense mantle of polygonal to rounded cells, delimited in lobes by abundant fibrovascular tissue. Moderate anisokaryosis and accentuated anisocytosis were observed and about 6 mitosis figures for every 10 high power fields (400x). The histological characteristics of the tumor were compatible with metastasis due to grade II solid mammary carcinoma. Immunohistochemical evaluations of anti-cytokeratin, anti-c-erbB2, and anti-estrogen and progesterone receptors were performed, with immunoreactivity for cytokeratin and c-erbB2 in neoplastic cells.
Discussion: There are no current data indicating which tumors that metastasize most frequently to the vertebral column. In the reported cases, it was found that the clinical signs presented by the animals are directly associated with the compression of specific areas of the spinal cord, as well as bone pain, resulting from tumor expansion and degeneration of the bone matrix, similar to what has been reported previously. The immunohistochemical diagnosis of anti-p63 in case 1 and anti-oncoprotein C-erbB2 in case 2, were essential to determine the neoplastic origin in the absence of the primary tumor. Despite being a place of low incidence of metastases, bones have a physiologically favorable environment for the implantation of neoplastic cells, especially when there is bone marrow involvement. Because of this and despite the rare occurrence, metastasis of carcinomas to the vertebral column should always be considered as differential diagnoses in patients presenting with clinical signs compatible with spinal cord compression and a history of previous neoplasia.


Download data is not yet available.

Author Biographies

Thierry Grima de Cristo, Universidade do Estado de Santa Catarina

Doutorando, Grupo de Patologia Veterinária

Laura Formighieri de Noronha, Universidade do Estado de Santa Catarina

Graduando em Medicina Veterinária

Giovana Biezus, Universidade do Estado de Santa Catarina

Doutorando, Grupo de Patologia Veterinária

Cinthya dos Santos Cerqueira, Núcleo de Anatomia Patológica, Instituto Adolfo Lutz


Natália Coelho Couto de Azevedo Fernandes, Núcleo de Anatomia Patológica, Instituto Adolfo Lutz


Gustavo Ribeiro Bonatto, Universidade do Estado de Santa Catarina

Graduando em Medicina Veterinária

Iliane Perin Arruda, Clínica Veterinária Cães e Gatos

Médica Veterinária

Renata Assis Casagrande, Universidade do Estado de Santa Catarina

Departamento de Medicina Veterinária


Alemayehu T. & Hailu D. 2019. Hepatocellular carcinoma with vertebral metastasis in a child with hepatitis B virus infection: A case report. Journal of Medical Case Reports. 13(1): 3-5.

Cassali G.D., Damasceno K.A., Bertagnolli A.C., Estrela-Lima A., Lavalle G.E., Di Santis G.W., De Nardi A.B., Fernandes C.G., Cogliati B., Sobral R., Costa F.V.A., Ferreira E., Salgado B.S., Campos C.B., D’Assis M.J.M.H., Silva L.P., Machado M.C.A., Firmo B.F., Nunes F.C. & Nakagaki K.Y.R. 2017. Consensus regarding the diagnosis, prognosis and treatment of canine mammary tumors: benign mixed tumors, carcinomas in mixed tumors and carcinosarcomas. Brazilian Journal of Veterinary Pathology. 10(3): 87-99.

Charney V.A., Miller M.A., Heng H.G., Weng H.Y. & Knapp D.W. 2017. Skeletal Metastasis of Canine Urothelial Carcinoma: Pathologic and Computed Tomographic Features. Veterinary Pathology. 54(3): 380-386.

Esposito M., Guise T. & Kang Y. 2018. The biology of bone metastasis. Cold Spring Harbor Perspectives in Medicine. 8(6): 1-7.

Goldschmidt M.H. & Goldschmidt K.H. 2017. Epithelial and Melanocytic Tumors of the Skin. In: Meuten D.J. (Ed). Tumors in Domestic Animals. 5th edn. Ames: John Wiley & Sons, pp.88-141.

Gundogdu F., Okur A., Atalay T., Tanik N. & Karacavus S. 2016. Multiple vertebral metastasis with fluid-fluid level from a pleomorphic lobular breast carcinoma. Spine Journal. 16(5): e303-304.

Gupta G.P. & Massagué J. 2006. Cancer Metastasis: Building a Framework. Cell. 127(4): 679-695.

Kallen M.E., Sanders M.E., Gonzalez A.L, Black J.O., Keedy V.L., Hande K.R., Homlar K.C., Halpern J.L., Holt G.E., Schwartz H.S., Coffin C.M. & Cates J.M.M. 2012. Nuclear p63 expression in osteoblastic tumors. Tumor Biology. 33(5): 1639-1644.

Marinho P., Ferrigno C., Bregadioli T., Paes F., Santos J.F., Macedo A.S., Dal-Bó Í.S. & Galeazzi V.S. 2018. Corpectomia lateral parcial para o tratamento de compressão ventral extradural crônica entre T9-T10 em um cão. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia. 70(5): 443-449.

Mumprecht V. & Detmar M. 2009. Lymphangiogenesis and cancer metastasis. Journal of Cellular and Molecular Medicine. 13(8): 1405-1416.

Nakagaki K.Y.R., Lima P.A., Utiumi K.U., Pires M.A.M., Zanatta R., Boabaid F.M., Colodel E.M. & Raymundo D.L. 2015. Transitional cell carcinoma of urinary bladder with metastasis in lumbar vertebrae and spinal cord compression in an ocelot (Leopardus pardalis). Pesquisa Veterinária Brasileira. 35(1): 75-79.

Pancotto T.E., Rossmeisl J.H., Zimmerman K., Robertson J.L. & Were S.R. 2013. Intramedullary spinal cord neoplasia in 53 dogs (1990-2010): Distribution, clinicopathologic characteristics, and clinical behavior. Journal of Veterinary Internal Medicine. 27(6): 1500-1508.

Peña L., Gama A., Goldschmidt M.H., Abadie J., Benazzi C., Castagnaro M., Díez L., Gärtner F., Hellmén E., Kiupel M., Millán Y., Miller M.A., Nguyen F., Poli A., Sarli G., Zappuli V. & De Las Mulas J.M. 2014. Canine Mammary Tumors: A Review and Consensus of Standard Guidelines on Epithelial and Myoepithelial Phenotype Markers, HER2, and Hormone Receptor Assessment Using Immunohistochemistry. Veterinary Pathology. 51(1): 127-145.

Rusbridge C., Wheeler S.J., Lamb C.R., Page R.L., Carmichael S., Brearley M.J. & Bjornson A.P. 1999. Vertebral Plasma Cell Tumors in 8 Dogs. Journal of Veterinary Internal Medicine. 13(2): 126-133.

Sharma N. & Purkayastha A. 2017. Follicular Thyroid Carcinoma Presenting as Large Solitary Vertebral Metastasis: Report of Two Unusual Cases Treated with Radiotherapy. Asia-Pacific Journal of Oncology Nursing. 4(2): 95-97.

Togawa D. & Lewandrowski K.-U. 2006. The Pathophysiology of Spinal Metastases. In: Mclain R.F., Markman M., Macklis R.M. & Benzel R.E.C. (Eds). Cancer in the Spine. 5th edn. Totowa: Humana Press Inc., pp.17-23.

Valentim L.G., Marcasso R.A., Bracarense A.P.F.L. & Arias M.V.B. 2018. Spinal Neoplasm in Dogs. Acta Scientiae Veterinariae. 44(1): 1-10.

Zhao Jie J.L., Gamaliel T.Y.H. & Hung W.P. 2018. Minimally Invasive Lateral Access Corpectomy for a Solitary Renal Cell Carcinoma Spinal Metastasis: A Case Report. Journal of Minimally Invasive Spine Surgery and Technique. 3(2): 70-74.



How to Cite

de Cristo, T. G., de Noronha, L. F., Biezus, G., Cerqueira, C. dos S., Fernandes, N. C. C. de A., Bonatto, G. R., Arruda, I. P., & Casagrande, R. A. (2020). Carcinoma with Vertebral Column Metastasis in Two Dogs. Acta Scientiae Veterinariae, 48.

Most read articles by the same author(s)