Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry

Authors

  • Everton Rafael Campos Federal University of Health Sciences of Porto Alegre
  • Sarah Eller Federal University of Health Sciences of Porto Alegre
  • Leticia Birk Federal University of Health Sciences of Porto Alegre
  • Masurquede Azevedo Coimbra Federal University of Health Sciences of Porto Alegre
  • Sandra Manoela Dias Macedo Federal University of Health Sciences of Porto Alegre; Instituto Nacional de Ciência e Tecnologia Forense
  • Mauricio Yonamine University of São Paulo
  • Josias Oliveira Merib Federal University of Health Sciences of Porto Alegre
  • Tiago Franco de Oliveira Federal University of Health Sciences of Porto Alegre https://orcid.org/0000-0003-2594-8415

DOI:

https://doi.org/10.22456/2527-2616.108853

Abstract

Anabolic-androgenic steroids (AAS) are synthetic derivatives of testosterone which are used medically for several diseases. However, misuse is commonly observed by athletes to promote enhancement of strength and performance. AAS are frequently obtained through online black markets from clandestine drug manufacturing laboratories, without any quality standards, being potentially dangerous for users. The purpose of this work was the development and application of a fast and simple procedure for the quantitation of stanozolol by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) in tablets packs seized in Rio Grande do Sul state, Brazil. The samples of stanozolol were separated considering its dosage form. The internal standard (methyltestosterone) was added to the aliquots of the samples, dissolved in methanol and 5μL were injected into the analytical system. The newly developed method has been validated for lower limit of quantitation (LLOQ), linearity, accuracy, precision and selectivity. The LLOQ was 0.1 µg/mL. The developed method was successfully applied to 31 samples seized by the Secretaria da Receita Federal do Brasil (a Brazilian federal revenue service agency). According to the results, 90.3% of the suspected medicines (n=31) were adulterated, and 65% exhibited higher concentrations of stanozolol than those indicated in the label. This work successfully established a new method for quantification of stanozolol using LC-QTOF-MS. This method aims at contributing to the identification and quantification of this anabolic androgenic steroid frequently seized by federal inspection agencies.

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Published

2020-12-18

How to Cite

Campos, E. R., Eller, S., Birk, L., Coimbra, M. A., Macedo, S. M. D., Yonamine, M., Merib, J. O., & Oliveira, T. F. de. (2020). Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry. Drug Analytical Research, 4(2), 58–63. https://doi.org/10.22456/2527-2616.108853

Issue

Section

ORIGINAL ARTICLES