Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant

Authors

  • Zakir Ullah Department of Animal Health & Department of Livestock Management, University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Hamayun Khan Department of Animal Health & Department of Livestock Management, University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Syean Maryam Hussain Department of Animal Health & Department of Livestock Management, University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Muhammad Tarique Tunio Department of Animal Health & Department of Livestock Management, University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Syed Muhmmad Raihan Dilshad Department of Animal Health & Department of Livestock Management, University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Ali Gohar Department of Animal Health & Department of Livestock Management, University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Hafsa Zahid Department of Animal Health & Department of Livestock Management, University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
  • Abid Ali Department of Animal Health & Department of Livestock Management, University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.

DOI:

https://doi.org/10.22456/1679-9216.90447

Abstract

Background: Addition of the antioxidant to extender media is the most appropriate attempt to reduce structural losses encounter during the process of cryopreservation. Hence semen excellence could be maintained for longer duration without adverse impact. Additionally antioxidants are not only capturing the reactive oxygen species but also improve the sperm quality indicators and fertility. Accordingly, current elucidation has been executed to explore the dose depended appraisal of varied concentration of α-tocopherol in Tris-based extender on frozen-thawed bull semen quality parameters for enhancement of bull semen cryopreservation in the subtropical ecosystem of Peshawar.

Materials, Methods & Results: Experiments were carried out on semen that has been collected from both Achai-an indigenous breed and Holstein Friesian (HF) - the exotic breed in artificial vagina maintained at 42°C from the experimental bulls of either breed and processed independently breed wise. Semen specimens with above 70% motility were evaluated separately breed-wise under same environmental condition. Standard procedure was adopted to extend the collected semen in the experimental extenders and frozen subsequently. After thawing, further Analysis of the frozen straws of semen was carried out for sperm excellence indicators that include motility, viability, acrosomal integrity and functional integrity of spermatozoa under the subtropical condition. Sperm viability and acrosomal integrity were determined by dual staining procedure i.e. Trypan-blue and Giemsa stains. The hypo-osmotic swelling (HOS) test was used to assess plasma membrane integrity. The current elucidation demonstrated that α-tocopherol 1.5 mg/mL supplemented in extender had significantly (P < 0.05) increased sperm excellence gauge that includes motility, viability, acrosomal integrity and functional membrane integrity in both the breeds. On the other hand, the result further elucidated those concentrations higher or lower than 1.5-1 mg/mL α-tocopherol supplemented in the present study resulted in a lower semen functional attributes subsequent to freezing.

Discussion: Biological samples were preserved by various methods such cryopreservation at low temperature to maintain them intact and facilitate downstream experiments. Antioxidants were associated with the chemical breakage of the substrate consequential from oxidation and counterbalance the free radicals thus diminish the damaging impacts to spermatozoa in cryopreservation. There is lack of data with respect to lipid peroxidation and cell reinforcement limit in cryopreserved semen, and cryopreservation is related with the generation of receptive oxygen species (ROS) which lead to lipid peroxidation (LPO) of sperm membranes, bringing about lost motility, viability and fertility of sperm. Recently, in the reproductive management of the dairy animals, an important focus of research is to overcome the deleterious impact associated with semen cryopreservation. Elucidation of differential dose depended expression of α-tocopherol in semen extender has been evident in this study. The results were corroborated with diverse mammalian species under varied ecological management setups. Furthermore the detrimental impact of high doses of α-tocopherol in the semen extender has been demonstrated. It was deduced from the current finding that addition of α-tocopherol via dose depended approach in Tri extender enhanced the cryosurvivability of both Achai indigenous breed and HF the exotic breed, under existing reproductive management in the local ecosystem of Peshawar.

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References

Aitken R.J., Harkiss D. & Buckingham D. 1993. Relationship between iron-catalyzed lipid-peroxidation potential and human sperm function. Journal of Reproduction Fertility. 98: 257-265.

Alkhedaide A., Alshehri Z.S., Sabry A., Abdel-Ghaffar T., Soliman M.M. & Attia H. 2016. Protective effect of grape seed extract against cadmium-induced testicular dysfunction. Molecular Medical Reproduction. 13: 3101-3109.

Amini Pour H., Tahmasbi A.M. & Naserian A.A. 2013. The influence of vitamin E on semen characteristics of ghezel rams in during cooling and frozen process. European Journal of Zoological Research. 2: 94-99.

Andrabi S.M.H., Ansari M.S., Ullah N. & Afzal M. 2008. Effect of non-enzymatic antioxidants in extender on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Pakistan Veterinary Journal. 28: 159-162.

Anghel A.H., S. Zamfirescu., Coprean D. & D. Nadolu. 2010. Antioxidant additives effect on cytological parameters of refrigerated ram semen. Lucrari. Science Journal. 53: 294-298.

Ball B.A., Medina V., Gravance C.G. & Baumber J. 2001. Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5°C. Theriogenology. 56: 577-589.

Beconi M.T., Francia C.R., Mora N.G. & Affranchino M.A. 1993. Effect of natural antioxidants on frozen bovine semen preservation. Theriogenology. 40: 841-851.

Bucak M.N., Ateşşahin A., Varişli O., Yüce A., Tekin N. & Akçay A. 2007. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen. Microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology. 67: 1060-1067.

Dorostkar K., Alavi-Shoushtari S.M. & Mokarizadeh A. 2012. Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis). Veterinary Research Forum. 3: 263-268.

Fonseca J.F., Torres C.A.A., Maffili V.V., Borges A.M., Santos A.D.F., Rodrigues M.T. & Oliveira R.F.M. 2005. The hypo-osmotic swelling test in fresh goat spermatozoa. Journal of Animal Reproduction. 2: 139-144.

Hashem N.M., El-Hady A.A. & Hassan O. 2013. Effect of vitamin E or propolis supplementation on semen quality, oxidative status and hemato-biochemical changes of rabbit bucks during the hot season. Livestock Science. 157: 520-526.

Hawkes W.C. & Turek P.J. 2001. Effects of dietary selenium on sperm motility in healthy men. Journal of Andrology. 22: 764-772.

Hu J.H., Zan L.S., Zhao X.L., Li Q.W., Jiang Z.L., Li Y.K. & Li X. 2010. Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen. Journal of Animal Science. 88: 1657-1662.

Jeyendran R.S., van der Ven H.H., Perez-Pelaez M., Crabo B.G. & Zaneveld L.J.B. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. Journal of Reproduction Fertility. 70: 219-228.

Kadirvel G., Kumar S. & Kumaresan A. 2009. Lipid peroxidation, mitochondrial membrane potential and DNA integrity of spermatozoa in relation to intracellular reactive oxygen species in liquid and frozen-thawed buffalo semen. Animal Reproduction Science. 114: 125-134.

Kamran D., Sayed Mortaza Alavi-Shoushtari. & Aram Mokarizadeh. 2012. Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis). Veterinary Research Forum. 4: 263-268.

Khan R.U., Rahman Z.U., Javed I. & Muhammad F. 2012. Effect of vitamins, probiotics and protein on semen traits in post-molt male broiler breeders. Animal Reproduction Science. 135: 85-90.

Kothari S., Thompson A., Agarwal A. & du Plessis S.S. 2010. Free radicals: Their beneficial and detrimental effects on sperm function. Indian Journal of Experimental Biology. 5: 425-435.

Moghbeli M., Kohram H., Zare-Shahaneh A., Zhandi M., Sharafi M., Nabi M.M., Zahedi V. & Sharideh H. 2016. Are the optimum levels of the catalase and vitamin E in rooster semen extender after freezing-thawing influenced by sperm concentration? Cryobiology. 72: 264-268.

Nasiri A.H., Towhidi A. & Zeinoaldini S. 2012. Combined effect of DHA and αtocopherol supplementation during bull semen cryoprese rvation on sperm characteristics and fatty acid composition. Andrologia. 44: 550-555.

Ordonez I. 2008. Systemic abnormalities by a deficiency of Vitamin E. Thesis. School of Dentistry. Universidad Nacional Autonoma de Mexico. 14: 29-39.

Pech-Sansores A.G., Centurión-Castro F.G., Rodríguez-Buenfil J.C., Segura-Correa J.C. & Aké-Lopez J.R. 2011. Effect of the addition of seminal plasma, vitamin E and incubation time on post-thawed sperm viability in boar Semen. Tropical and Subtropica Agroecosystem. 14: 965-971.

Raina V.S., Gupta A.K., & Singh K. 2002. Effect of antioxidant fortification on preservability of buffalo semen. Asian-Australian Journal of Animal Science. 15: 16- 18.

Saleem M., Rahim I., Rueff H., Khan M., Maselli D. & Wiesmann U. 2010. Mountain cattle breed for coping with climate change: needs for conserving and reintroducing the Achai in the Hindu Kush Mountains of Northern Pakistan. Conference on International Research on Food Security, Natural Resource Management and Rural Development Tropentag ETH Zurich. September 14-16. 4p.

Schäfer-Somi S., Kluger S., Knapp E., Klein D. & Aurich C. 2006. Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa. Theriogenology. 66: 173-182.

Seremak B., Udala J. & Lasota B. 1999. Influence of selenium additive on ram semen freezing quality. Electronic Journal of Polish Agricultural Universities. 2: 1-5.

Silva P.F.N. & Gadella B.M. 2006. Detection of damage in mammalian sperm cells. Theriogenology. 65: 958-978.

Uc N., Centurión F., Alfaro M., Rodríguez. J. & Sarmiento L. 2010. The addition of αtocopherol and ascorbic acid to the freezing media and their effects on viability of post thawed swine semen. Reproduction in Domestic Animals. Abstract 45:18

Wahjuningsih S. & Rachmawati A. 2012. The Effect of a-tocopherol on plasma membrane integrity of goat spermatozoa. Journal of Basic and Applied Science Research. 2: 8857-8860.

Published

2019-01-01

How to Cite

Ullah, Z., Khan, H., Hussain, S. M., Tarique Tunio, M., Raihan Dilshad, S. M., Gohar, A., Zahid, H., & Ali, A. (2019). Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant. Acta Scientiae Veterinariae, 47(1). https://doi.org/10.22456/1679-9216.90447

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